Dynamics of splenic germinal center development in macaques infected with <i>Leishmania infantum</i>.

<p>(<b>A</b>–<b>D</b>) Splenic tissue sections were stained with antibodies against Ki-67 (white), IgD (green), CD3 (blue) and CD20 (red) and imaged by confocal microscopy. Shown are representative pictures of a naïve animal (A) and at 11 (B), 28 (C) and 250 (D) days after infection. (<b>E</b>) Splenic tissue sections were stained with an antibody against Ki-67 by immunohistochemistry and germinal centers were counted in naïve macaques (n = 3), and at days 11 (n = 2), 28 (n = 2) and 250 (n = 4) after infection. Three distinct sections per animal were examined. Bars depict mean ± SEM. (<b>F</b>) Quantification of GC area in Ki-67-stained splenic tissue sections from naïve and infected macaques at the indicated time points. Statistical analysis was performed by one-way ANOVA, followed by a Bonferroni's post-hoc test.