Validation of Venlafaxine in Pharmaceutical Dosage by Reverse Phase HPLC

<p>A rapid, specific and accurate isocratic HPLC method was developed and validated for the assay of venlafaxine in pharmaceutical dosage forms. The assay<br>involved an isocratic – elution of venlafaxine in ODS- C18 column using mobile phase composition consists of (65:35 v/v) of acetonitrile and sodium acetate<br>with 0.1% tri-ethyl amine. The wavelength of detection is 225nm.The method showed good linearity in the range of 2.0-50.0 mg/mL. The runtime of the<br>method is 5 mins. The proposed method can be used for routine quality control samples in industry in bulk and in finished dosage forms. In present study,<br>a rapid specific precise and validated HPLC method for the quantitative estimation of venlafaxine in pharmaceutical dosage forms has been reported. The<br>developed method can be applied to directly and easily to the analysis of the pharmaceutical tablet p reparations. The percentage recoveries were near<br>100% for givenmethods. The method was completely validated and proven to be rugged. The excipients did not interfere in the analysis. The results showed<br>that this method can be used for rapid determination of venlafaxine in pharmaceutical tablet with precision, accuracy and specificity.</p> <p>