A_2AR is expressed in rat postnatal IPL and GCL.

<p>A–B. Immunofluorescence staining of (A) the adenosine A_2A receptor (A_2AR) and (B) choline acetyltransferase (ChAT) in retinal cross-sections from P2 rats. C. The merged image of the A_2AR (green) and ChAT (red) staining. The cell nuclei were stained with DAPI (blue). NBL, neuroblast layer; IPL, inner plexiform layer; GCL, ganglion cell layer. D. The high magnification of the merged image in the box of C. The arrow indicated a starburst amacrine cell (SAC). E. Immunofluorescence staining of A_2AR (green) and ChAT (red) in single SACs dissociated from the P2 rat retinas. <i>Right</i>, the merged image under the bright field. The colocalization signals were shown in yellow. Scale bars for A–C, 50 µm. Scale bar for D, 5 µm. Scale bar for E, 7.5 µm.