Mycosomes from egg yolk revert to walled cells.

<p>(a–b) Yolk-Ms show prolific reproduction within thin membrane sacs. (a) Phase contrast, (b) SYTO 9 stain. (c) IAA-treated Ms formed large membrane-sacs containing numerous spheroid protoplasts, each with a prominent central body. (d–e) Ms cultured in MsM flooded over Sabouraud dextrose agar. The variable-shaped lipoid bodies (d) contain (e) fluorescing PMOs or Ms (SYTO 9 stain). (f–k) Cell wall formation by <i>Rhodotorula glutinis</i> stained for chitin with Fungi-Fluor. (f and h) Yeast cells were present at 38 days within a diffuse protoplast matrix containing lipoid-Ms (phase contrast). (g and i) Ms and the protoplast matrix do not express chitin; only the mature cell (g) and thin walled yeast cells (i) fluoresce. (j–k) Sampled two weeks later, three walled cells and many enlarging Ms show variable cell wall fluorescence. Bars = 2.0 µm.