Mean area of CNV significantly increased at day 14 after photocoagulation.

<p>16–20 week-old female C57BL/6 mice were given laser photocoagulation spots at 3, 12 and 9 o'clock positions 1PD away from the optic disc in the posterior pole of retina of each eye with a cover slide placed on the cornea as a contact lens. After 5, 7, 9, 11, 14, 17, 21 days, mice were perfused with fluorescein-labeled dextran and choroidal flat mounts were examined by fluorescence microscopy. The mean (±SEM) area of CNV was calculated from each experimental value of each laser spot. CNV appeared from day 5 (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0094743#pone-0094743-g005" target="_blank">Fig. 5A</a>), increased significantly at day 14 (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0094743#pone-0094743-g005" target="_blank">Fig. 5E</a>, P = 0.017), and decreased thereafter. Significant differences were observed of day 14 from any other point except for day11, but no significant difference was detected between day11 and any other day point <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0094743#pone-0094743-g005" target="_blank">Figure 5A to 5G</a> show choroidal representative flat mounts of mice euthanized 5, 9, 11, 14, 17, 21 days after photocoagulation at 100× magnification. The laser spots are shown by arrows. Statistics were analyzed by one-way ANOVA with Student-Newman-Keuls method for multiple comparisons. (n = 6mice/group, *P<0.05).