Identification of domains in MBNL1 responsible for regulation of alternative splicing of endogenous (A–B) <i>SERCA1</i> exon 22, (C–D) <i>IR</i> exon 11 and (E) <i>NFIX</i> exon 7.

<p>RT PCR splicing assay results for inclusion/exclusion levels of respective exons upon over-expression of FL-MBNL1, its deletion mutants or empty vector/Mock in (A,C,E) HEK293T and (B and D) DM1 myoblasts. At least four independent transfections were done for each construct. Relative fold change compared to Mock for inclusion/exclusion levels of respective exons is shown. Error bars denote standard deviation (SD) on the graphs. One-way ANOVA with Tukey's multiple comparison method for statistical significance was performed. Means that do not share a letter are significantly different.