<p>The RT-PCR analysis of corneal RNA was performed as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0096435#pone-0096435-g001" target="_blank">Fig. 1</a> legend. For primers located within a single exon PCR was performed with (+) and without (–) the RT step. For primer pairs located in separate exons only results of PCR with (+) the RT are shown. Arrows indicate the positions of the predicted PCR fragment sizes for <i>Taar4</i> (140 bp), two splice variants of Gαolf (314 and 607 bp), <i>Gna15</i> (118 bp), <i>Reep5</i> (347 bp), <i>Rtp3</i> (180 bp), <i>Omp</i> (150 bp) and <i>Emx2</i> (133 bp). Molecular markers (M) are from a 100 bp DNA ladder; the lowest band is 100 bp.</p
<p>For each of the 355 OR genes (rows), the RNA copies number were estimated from normalized relativ...
<p><b>Copyright information:</b></p><p>Taken from "Expression and Localization of the Cell Adhesion ...
Amplicons for were visualized in a 1.5% agarose ethidium bromide gel from both samples KC and non-K...
<p>RT-PCR was performed as described in Materials and Methods using mouse corneal RNA and primers fo...
<p>RT-PCR – the expression of the gene was (yes) or was not (no) confirmed by RT-PCR, or not tested ...
<p>Predicted exons –number of gene exons predicted by bioinformatics methods.</p><p>Found exons –num...
<p>Corneal RNA was isolated and subjected to qPCR as described in Materials and Methods. PCR product...
To advance our understanding how the outer eye interacts with its environment, we asked which cellul...
<div><p>Purpose</p><p>To advance our understanding how the outer eye interacts with its environment,...
PCR products from both samples KC and non-KC corneal tissue are shown. Curves A and B correspond to ...
<p>Reverse transcription-PCR results showing the detection of various members of Rho proteins in hum...
PURPOSE. To increase the database of genes expressed in human cornea and to gain insights into the m...
Purpose: To evaluate the suitability of common housekeeping genes (HKGs) for use in quantitative rev...
<p>Specific primers of forty-seven putative OBP genes were used in non quantitative RT-PCR experimen...
<p>A) RT-PCR reactions were performed with 50 ng of RNA extracted from an overnight TGY culture of t...
<p>For each of the 355 OR genes (rows), the RNA copies number were estimated from normalized relativ...
<p><b>Copyright information:</b></p><p>Taken from "Expression and Localization of the Cell Adhesion ...
Amplicons for were visualized in a 1.5% agarose ethidium bromide gel from both samples KC and non-K...
<p>RT-PCR was performed as described in Materials and Methods using mouse corneal RNA and primers fo...
<p>RT-PCR – the expression of the gene was (yes) or was not (no) confirmed by RT-PCR, or not tested ...
<p>Predicted exons –number of gene exons predicted by bioinformatics methods.</p><p>Found exons –num...
<p>Corneal RNA was isolated and subjected to qPCR as described in Materials and Methods. PCR product...
To advance our understanding how the outer eye interacts with its environment, we asked which cellul...
<div><p>Purpose</p><p>To advance our understanding how the outer eye interacts with its environment,...
PCR products from both samples KC and non-KC corneal tissue are shown. Curves A and B correspond to ...
<p>Reverse transcription-PCR results showing the detection of various members of Rho proteins in hum...
PURPOSE. To increase the database of genes expressed in human cornea and to gain insights into the m...
Purpose: To evaluate the suitability of common housekeeping genes (HKGs) for use in quantitative rev...
<p>Specific primers of forty-seven putative OBP genes were used in non quantitative RT-PCR experimen...
<p>A) RT-PCR reactions were performed with 50 ng of RNA extracted from an overnight TGY culture of t...
<p>For each of the 355 OR genes (rows), the RNA copies number were estimated from normalized relativ...
<p><b>Copyright information:</b></p><p>Taken from "Expression and Localization of the Cell Adhesion ...
Amplicons for were visualized in a 1.5% agarose ethidium bromide gel from both samples KC and non-K...