Both PPO1 and PPO2 contribute to injury related melanization in larvae.

<p>(<b>A</b>) Melanization of larvae after clean injury is abolished in the simultaneous absence of PPO1 and PPO2. A reduced melanization spot was observed in <i>PPO1</i>^Δ mutants. <i>PPO2</i>^Δ mutant larvae displayed a more intense melanization at the wound site when compared to wild-type. Arrows indicate the pricking site. Larvae were wounded with a tungsten needle and blackening of the wound was recorded 30 minutes later. A representative picture is shown for each genotype. (<b>B</b>) <i>PPO1</i>^Δ larvae have a defect in melanization rate upon pricking compared to wild-type and <i>PPO2</i> mutant larvae. <i>Cantons^S</i> larvae were used as an additional wild-type control. Larvae were wounded with a tungsten needle and the presence of a blackening wound was recorded every 5 minutes up to 30 minutes. Data were analyzed using the Log rank test and values represent the mean±s.e. of at least three independent experiments of 10 larvae each.