Impact of SREC-I on adhesion of <i>S. aureus</i> to HNECs.

<p>Inhibition of SREC-I dependent adhesion to HNECS by Fab₂- fragments of an anti-human SREC-I antibody in 24 well microtiter plate assays under static conditions (A) and shear stress conditions in ibidi chamber slide assays (B). 10 µg/ml of the anti-SREC-I Fab₂-fragments and isotype control were used in all assays. Attachment of the <i>S. aureus</i> wild-type (wt), and <i>tagO</i> mutant was evaluated microscopically by counting at least 6 visible fields per well or chamber slide containing HNECs. The data is expressed as the means and SEM of 5 independent experiments under static and flow conditions. Statistical analysis was performed by one-way ANOVA with Bonferroni's multiple comparison test (A and B). Fab₂- fragments of an anti-human SREC-I antibody were also used to test the influence of SREC-I on bacterial adhesion under flow condition to CRNECs in ibidi chamber slide assays (C). 10 µg/ml of the SREC-I Fab₂-fragments and isotype control were used in all assays. Attachment of the <i>S. aureus</i> wild-type (wt), and <i>tagO</i> mutant was evaluated microscopically by counting at least 6 visible fields per chamber slide containing CRNECs. The data represent mean and SEM of 5 independent experiments. Statistical analysis was performed by one-way ANOVA with Bonferroni's multiple comparison test. (C) We also tested the influence of SREC-I on the adhesion of other <i>S. aureus</i> strain backgrounds (D). Fab₂- fragments of an anti-human SREC-I antibody were used under flow conditions in ibidi chamber slide assays with HNECS. 10 µg/ml of the SREC-I Fab₂- fragments and isotype control were used in all assays. Attachment of the <i>S. aureus</i> Newman (wt), and SH1000 mutant was evaluated microscopically by counting at least 6 visible fields per chamber slide containing CRNECs. The data represent mean and SEM of 4 independent experiments. Statistical analysis was performed by one-way ANOVA with Bonferroni's multiple comparison test. (D) Significant differences between groups are indicated by one (<i>P</i><0.05), two (<i>P</i><0.01), or three (<i>P</i><0.001) asterisks (<b>*</b>).