ERM-1 negatively regulates vulval induction and binds to LET-23.

<p>(A) Genetic epistasis analysis between <i>erm-1</i> and components of the <i>egfr/ras/mapk</i> pathway. Vulval induction (VI) indicates the average numbers of induced VPCs in different double mutant combinations scored in <i>erm-1(tm677)</i> heterozygous (white bars) versus homozygous (gray bars) animals. %Vul indicates the fraction of animals with VI<3 and % Muv the fraction of animals with VI>3. The numbers of animals scored for each genotype are indicated in brackets. N.S: no significant change. *Indicates p<0.05 as determined in a two tailed student's t-test - two-sample unequal variance. (B) Structures of the GST::ERM-1 fusion proteins tested for LET-23 binding. (C) Interaction of LET-23 from wild-type extracts with different GST::ERM-1 fusion proteins detected on an anti-LET-23 Western blot. (D) Binding of LET-23 extracted from <i>lin-7(e1413)</i> and (E) from <i>let-23(sy1)</i> mutants to GST::ERM-1 proteins. The dashed lines indicate the approximate positions of the 180 kDa and 116 kDA protein standards.