SsnB inhibits cell growth and viability of neuroblastoma cells.

<p>(A) Phase contrast images showing the morphology of neuroblastoma cells after treatments with SsnB. Neuroblastoma cells (SH-SY5Y, IMR-32, NGP, SKNF-1 and SK-N-BE(2) cells) were grown in presence of DMSO or SsnB (1, 5, 10, and 20 µM) in complete culture medium and were photographed on indicated times. (<b>B</b>) Bar diagrams showing the cell viability after SsnB treatment as evaluated by MTT assays. Neuroblastoma cells were treated with SsnB (1, 5, 10, and 20 µM) for 2 days (SH-SY5Y and IMR-32), 3 days (NGP) or 4 days (SKNF-1 and SK-N-BE(2) cells) and cell viability was measured by MTT assay at 575 nm. Data are represented in fold change and *<i>p</i><0.05 <i>vs</i> control. (<b>C</b>) SsnB arrest cell cycle at G2/M phase. Representative histograms illustrating the cell cycle progression of neuroblastoma cells in presence of SsnB. Neuroblastoma cells treated with SsnB (1 µM or 10 µM) in DMEM with 10% FBS for 2 days (SH-SY5Y and IMR-32), 3 days (NGP) or 4 days (SKNF-1 and SK-N-BE(2) cells) were labelled with propidium iodide and cell cycle stage was analyzed by flow cytometry.