Evidence for functional variation at identified genes.

<p>In A, we show representative results from genetic engineering experiments in a multi-locus introgression strain. Haploid deletion strains are shown in the top row, while the second and third rows contain diploid hemizygotes. For each gene and allele, we constructed multiple hemizygotes, with representative phenotypes shown in the figure. The terms ‘causal’ and ‘non-causal’ refer to which allele was detected in our initial genetic mapping experiment. In B, we used genetic engineering in a smooth backcross segregant with the genotype <i>END3</i>^BY<i>FLO8</i>^3S<i>IRA2</i>^BY<i>MSS11</i>^BY<i>TRR1</i>^3S to confirm the involvement of <i>IRA2</i> in rough colony morphology. The segregant subjected to transformations is shown on the left, followed by representative knock-ins of <i>IRA2</i>^BY, <i>IRA2</i>^3S, and <i>IRA2</i>^3S-Δ2933. The allele replacements have a <i>kanMX</i> tail, which was used to select for integration into the chromosome.