Progression of wound healing.

<p>A. Wound model. The scheme shows the four back injuries in a Wistar rat model, comprising the dermis and epidermis layers. The treatments were, in the upper left injury (1): 100 µL of CMC 20 mg/mL+26×10⁶ of ADSCs; upper right (2): 100 µL CMC 10 mg/mL+26×10⁶ of ADSCs; bottom left (3): 100 µL of CMC 20 mg/mL; bottom right (4): Sham. B. The figure is showing the image of one animal, representative of each group, from day 0 to 16, where the closure of wounds was followed and documented daily. Photographs in the left column were taken in day zero, immediately after surgery. In the right column, in the euthanasia day. C. Evaluation of the area of lesions. The graphic shows, in cm², the difference between the size of the initial lesion and the size after different days of healing. There was no difference among the groups. D. Determination of epithelium thickness. Values are expressed in micrometers (µM) and demonstrate a significant increase in the epithelium thickness in the group of animals treated with 10 mg/mL of CMC + ADSCs (lesion 2), with eight days of healing, when compared with control group (injury 4, Sham). (*P<0.05, two-way ANOVA test). CMC: Sodium carboxymethylcellulose; ADSC: Adipose-derived stem cell; Sham: Without treatment.