Effect of glatiramer acetate (GA) on platelet adhesion to collagen and aggregation under flow conditions.

<p>Whole mouse blood samples were collected from normal C57BL/6 mice using sodium citrate as anticoagulant and pretreated with PBS or GA (50 or 100 µg/ml) for 30 min as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0096256#s5" target="_blank"><i>Methods</i></a>. After pretreatment with PBS or GA, the whole blood samples were labeled with platelet-specific antibodies and then perfused through the camber with collagen coated glace slides as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0096256#s5" target="_blank"><i>Methods</i></a>. Collagen coated slides with aggregated pre-labeled platelets were washed, fixed and subsequently analyzed using fluorescence microscopy. Representative immunofluorescent images for samples treated with PBS or GA (50 or 100 µg/ml) are shown in (<b>A</b>). Average size of thrombi ± S.E. is shown in (<b>B</b>).