Acrylamide exposure elicits an increase in CYP gene expression in the male germ line.

<p>Gene expression levels were analysed in isolated spermatocytes by Q-PCR following incubation with acrylamide. Cells were isolated and cultured with acrylamide (1 uM, 18 h), RNA was extracted, reverse transcription performed and QPCR conducted as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0094904#s2" target="_blank">Materials and Methods</a>. CYP2E1 and CYP1B1 gene expression was significantly increased in spermatocytes following acrylamide exposure (<i>F</i>_1,16 = 20.1, *<i>p</i><0.05). Data are depicted as transformed values (2e−ΔΔC(t)) as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0094904#s2" target="_blank">Materials and Methods</a>, and is representative of n = 3 experiments (Mean ±SEM).