Acrylamide is metabolized in spermatocytes to glycidamide via CYP2E1.

<p>The Isolated spermatocytes were treated with acrylamide (1 µM, 18 h), glycidamide (0.5 µM, 18 h), resveratrol (0.1 µM, 18 h), or a combination of acrylamide (1 µM) and resveratrol (0.1 µM, 18 h) or glycidamide (0.5 µM) and resveratrol (0.1 µM, 18 h). DNA damage was assessed by the comet assay modified by the addition of the FPG or hOGG1 enzyme. Significant levels of DNA damage were detected in the acrylamide treated spermatocytes in the presence of FPG (<i>F</i>_13,877 = 92.5, ***<i>p</i><0.001) or hOGG1 (**<i>p</i><0.001). A significant increase in DNA damage was observed with glycidamide treatment in the presence of FPG (***<i>p</i><0.001). Resveratrol treatment on its own had no effect on the level of DNA damage in spermatocytes with FPG or hOGG1 treatment. DNA damage assessed in cells treated with the combination of acrylamide and resveratrol was not significantly different from control. Treatment with glycidamide and resveratrol caused a significant induction in DNA damage when treated with FPG (***<i>p</i><0.001), but not hOGG1. Spermatocytes treated with H₂O₂ (500 µM, 5 min) were used as a positive control for DNA damage. Significant increases in Tail DNA % were observed in cells treated with H₂O₂ in the presence of either FPG or hOGG1 (***<i>p</i><0.001). All data are representative of n = 3 experiments (Mean ±SEM).