Gene expression of CYP2E1 in the male germ line.

<p>Q-PCR analysis of CYP2E1 mRNA expression in mouse testis at different developmental stages 2, 6, 11, 14, 18, 22, and 36 d after birth, and adult (older than 56 d). Expression was also examined in isolated male germ cells, spermatogonia, spermatocytes and spermatids. Data are representative of n = 3 experiments and depicted as transformed values, 2e−ΔC(t) (Mean ±SEM), as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0094904#s2" target="_blank">Materials and Methods</a>. CYP2E1 gene expression was found predominantly in spermatogonia. Statistically significant differences were found in spermatogonia, 11, 14 and 18 d compared to 2 d testis (<i>F</i>_10,79 = 4.0,*<i>p</i><0.05).