Loss of Tet2 accentuates a Kit D814V driven mast cell phenotype.

<p><b>A</b>) Average number of mast cells per skin section across genotypes. N = 60–80 sections from 3–4 independent animals/genotype. *<i>P</i><.05. <b>B</b>) Average number of mast cells per stomach/esophagus section across genotypes. N = 60–80 sections from 3–4 independent animals/genotype. *<i>P</i><.05. For Figure 2A and 2B, numbers 1–4 indicate the following genotypes: 1 = WT ctr, 2 = Tet2^+/+;Kit D814, 3 = Tet2^−/−;Kit D814, 4 = Tet2^−/−;Kit WT. <b>C</b>) Percentage of skin sections with a defined histology score from Tet2^+/+;Kit D814V and Tet2^−/−;Kit D814V. <b>D</b>) Percentage of stomach/esophagus sections with a defined histology score in Tet2^+/+;Kit D814V and Tet2^−/−;Kit D814V animals. For Fig 2A–2D, twenty randomly chosen and independent regions of equal thickness per animal were counted in a blinded fashion at 20× magnification, and scored according to the classification reported in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0096209#pone-0096209-t001" target="_blank">Table 1</a>. Mice were all harvested between 8 and 20 weeks after the last pI:C injection. n = 4 per genotype. <b>E</b>) Representative pictures of Giemsa staining performed on skin (left panels) or stomach/esophagus sections (right panels) prepared from Tet2^+/+;Kit D814V and Tet2^−/−;Kit D814V animals. Mast cells stain dark blue in these sections. Scale bar represents 100 µm.