Expression of Kit D814V and loss of Tet2 in MC partially recapitulates the Mx1-Cre-mediated phenotype.

<p><b>A</b>) Cutaneous mastocytosis in mice expressing Kit D814V under the control of a mast cell-specific Cre (Mcpt5-Cre) promoter. Graph represents the average number of mast cells/skin section across listed genotypes. N = 56–96 from 3–4 independent animals per genotype. ns = not significant. Numbers 1–6 indicate the following genotypes: 1 = WT ctr, 2 = Tet2^+/+;Kit D814;Mcpt5-Cre, 3 = Tet2^+/−;Kit D814;Mcpt5-Cre, 4 = Tet2^−/−;Kit D814V;Mcpt5-Cre, 5 = Tet2^+/−;Kit WT;Mcpt5-Cre,6 = Tet2^−/−;Kit WT;Mcpt5-Cre, <b>B</b>) Histological score of disease in affected Mcpt5-Cre animals. Each skin region was scored according to the criteria reported in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0096209#pone-0096209-t001" target="_blank">Table 1</a>. Bar graph represents the percentage of sections per genotype with a defined histology score (from 3 to >4). Mice analyzed were 9-month-old (n = 3–4 per genotype). <b>C</b>) Representative microphotographs showing mast cell infiltration in Tet2^+/+; Kit D814V;Mcpt5-Cre (dermal lymph node), Tet2^+/−;Kit D814V;Mcpt5-Cre and Tet2^−/−;Kit D814V;Mcpt5-Cre animals (skin sections). Mast cells stain dark blue in these sections. Scale = 100 µm.