MiRNA-30c transgenic mice have a mitochondrial phenotype.

<p>(a) Western blot of mitochondrial OXPHOS proteins from wildtype and transgenic littermates, before the onset of the phenotype, at 4 weeks of age in line B. (b) Quantification of western blots of mitochondrial OXPHOS proteins of panel A (N = 6). (c) Western blot of mitochondrial OXPHOS proteins in line B at 10 weeks of age, after DCM starts to develop. (d) Overview images of electron microscopy from wildtype (left) and miRNA-30c TG mice (right) of line B. Mitochondria are indicated with a black arrowhead. (e) Quantification of mitochondrial area (N = 3, 18 images per heart) at 4 weeks of age. (f) Quantification of mitochondrial to genomic DNA ratio by qPCR (N = 4) at 4 weeks of age in line B. (g) Mitochondrial citrate synthase activity at 4 weeks of age (N = 4) in line B. Error bars represent s.e.m. and * denotes a p-value ≤0.05.