Effect of SKI-II on Nrf2 in primary human airway epithelial cells and in mice <i>in vivo</i>.

<p><b>A.</b> Whole-cell extracts from normal human bronchial epithelial cells (HBEC) treated with increasing concentrations of SKI-II (0.1 to 3 µM) for 2 h were analysed by immunoblotting for Nrf2 and normalized using β-actin. ** p<0.001, * p<0.05. <b>B.</b> Gene expression of NQO1, GCLM and HO-1 were determined after 24 h treatment with SKI-II (1 µM) in HBEC. GNB2L1 was used as housekeeping gene. *** p<0.0001, * p<0.05. <b>C, D, E, F</b> and <b>G.</b> A/J mice were exposed to nebulized SKI-II (10 µM in PBS, n = 3) or vehicle (Veh, n = 3) for 2 h followed by a further 2 h exposure. Whole-cell extracts were analysed by immunoblotting for Nrf2, GCLM, HO-1 and NQO1 and normalized using β-actin expression. Results are representative of 3 independent experiments and are means and S.E. of triplicates.