Effect of siUL19 on plaque formation and relative <i>UL19</i> gene expression of acyclovir-resistant HSV-1 strains and HSV-2 333.

<p>(A–D) Effects of siUL19 on relative mRNA expression level of the <i>UL19</i> gene were analyzed by qPCR compared with the viral group (HSV-2/33, HSV-2/106, HSV-1/153 and HSV-1/Blue, respectively). (a–d) Effects of siUL19 on proliferation of acyclovir-resistant HSV-1 and HSV-2 333 were evaluated by a plaque reduction assay. Cells were treated with siUL19 and infected with each virus (HSV-2/33, HSV-2/106, HSV-1/153 and HSV-1/blue, respectively). Plaques were counted 72 h later, and the relative survival rate of each group was compared with virus control set at 100%. Each sample was analyzed in triplicate, and data are expressed as the mean ± SEM. *<i>P</i><0.05 vs. viral group.