C/EBPβ-2 induces <i>galectin-7</i> mRNA levels in breast cancer cell lines.

<p>(<b>A</b>) RT-PCR analysis showing increased expression of <i>galectin-7</i> in human breast cancer cells after transfection with an expression vector encoding C/EBPβ-2. The two lanes represent two different samples. No such increase was observed in cells transfected with an expression vector encoding C/EBPβ-3. Similar results were obtained with HaCaT cells, a keratinocyte cell line which constitutively expresses <i>galectin-7</i>. An empty pCMV5 vector was used as transfection control (CTRL) and GAPDH was used as loading control. (<b>B</b>) RT-PCR analyses showing expression of <i>galectin-7</i> mRNA levels in MCF-7 cells after transfection with increasing doses of an expression vector encoding C/EBPβ-2. GAPDH was used as loading control. (<b>C</b>) RT-PCR analysis of MCF-7 cells co-transfected with vectors encoding C/EBPβ-2 and C/EBPβ-3. Below, control Western blot analysis showing expression of C/EBPβ-2 and C/EBPβ-3 after transfection. β-actin was used as loading control.