Blockade of TGF-β <i>in vivo</i> by ApoLinkerP144 delivered either by gene therapy or bound to HDL.

<p>(A) Western blot against ApoA-I with purified HDL fractions of plasma obtained from animals which received 24 h before either pApo or pApoLinkerP144 by hydrodynamic injection. (B) Inhibition of TGF-β by HDL from mice injected with Apo plasmid (HDL Apo) or HDL containing ApoLinkerP144 (HDL ApoLinkerP144) measured using Mv-1-Lu cells cultured in the presence of 200 pg/ml of TGF-β and 10 µg/ml HDLs. Mean±SEM *, <i>P</i><0.05 (C) Strengthened pStat-1 expression and diminished pSmad-2 expression in livers from mice which received IL-12 plasmid and HDL Apo or HDL ApoLinkerP144. Numbers indicate the relative intensity of the bands. (D) IFN-γ production 4 days after IL-12 plasmid hydrodynamic injection combined with i.p injection of HDL Apo or HDL ApoLinkerP144 or a hydrodynamic injection of pApoLinkerP144 in C57BL/6 mice (<i>n</i> = 4 mice/group). Mean±SEM **, <i>P</i><0.01.