Podoplanin transcript and protein expression levels in differentiated thyroid cancer derived cell lines.

<p>A. PDPN mRNA expression in human thyroid cancer cell lines. RT-qPCR was used to evaluate levels of the transcript encoding podoplanin in total RNA prepared from NTHY control cells, PTC-derived TPC1 and BcPAP cell lines, and FTC-derived FTC133 and CGTH-W-1 cell lines. Data from four independent experiments performed in triplicate are expressed as the mean ±SEM, **<i>P</i><0.001. B. Podoplanin protein levels in human thyroid cancer cell lines. Protein extracts (30 µg) were subjected to Western blot analysis with anti-PDPN monoclonal antibody D2-40 and β-actin antibody as a loading control. C. Immunofluorescence analysis of the podoplanin expression in TPC1, BcPAP, FTC133 and CGTH-W-1 thyroid cancer cell lines. PDPN was visualized by staining using anti-PDPN monoclonal antibody D2-40 followed by DyLight549-conjugated secondary antibody (red), and nuclei were counterstained with DAPI (blue). Magnification 1000x.