JMJD3 interacts with p53.

<p>a, The number of JMJD3, UTX and p53 peptides identified by mass spectrometry in the tandem anti-Flag-HA purifications of Flag–HA–JMJD3 and Flag-HA-UTX stably expressed in HEK293 cells. <b>b</b>, Phoenix cells were transfected with HA-JMJD3, HA-UTX or an empty vector (Emp), and immunoprecipitated with an antibody against the HA-tag. Western blotting was performed with antibodies against HA or p53. <b>c</b>, Co-immunoprecipitation of endogenous JMJD3 and p53 was performed in Phoenix cells by immunoprecipitating with HA (negative control) or JMJD3 antibody. <b>d</b>, Schematic representation of wild type (wt) p53 and the three p53 deletion mutants lacking the regulatory (REG), the tetramerization (TET) or the transactivating (TA) domains as indicated. <b>e</b>, Pheonix cells were transfected with myc-tagged JMJD3 alone or together with wt p53 or p53 deletion mutants as indicated. Immunoprecipitation was performed with myc- or HA antibody followed by western blotting.