<i>S</i>. Typhimurium does not require the glyoxylate shunt or gluconeogenesis for intracellular replication within HeLa cells.

<p>Invasion (A) and intracellular replication (B) of <i>S</i>. Typhimurium 4/74, Δ<i>aceA</i> (JH3385), and Δ<i>pps</i>Δ<i>pckA</i> (JH3487) strains during infection of HeLa cells. (A) The chart shows the numbers of viable bacteria (expressed as percentages of the initial inoculum) within host cells at 2 h after infection. (B) The chart shows the percentage replication of bacteria between 2 h and 6 h. Each bar represents the statistical mean from two biological replicates (performed in triplicate) and the error bars represent the standard deviation.