PKC, ERK/MAPK, CAMKII and intracellular calcium release negatively regulate Rv3416 mediated VGCC expression.

<p>PMA stimulated THP1 cells were incubated with inhibitors to indicated molecules (<i>see Experimental Procedures</i>) for 1h prior to stimulation with Rv3416. L-type VGCC levels were monitored by FACS 72h post stimulation. Bold lines represent cells stimulated with 30 µg/ml of Rv3416, while dotted lines represent cells incubated with inhibitors to indicated molecules followed by stimulations with Rv3416. Thin lines represent unstimulated cells. One of four independent experiments is shown.