The determination of isoflurane enantiomers' binding to the αL I domain using 1-aminoanthracene (1-AMA).

<p>(A) The reduction of fluorescence intensity versus isoflurane concentration after displacement of 1-AMA from the αL I domain. Error bars indicate standard deviation (S.D.) of means of three independent experiments. (B) First, Kd values of S- and R-isoflurane in each independent experiment was obtained. Then the average Kd values of S- and R-isoflurane of three independet experiments were calculated (S-isoflurane 374.8 µM, R-isoflurane 460.2 µM). Data represent mean +/- S.D. F test was used for statistical analysis. No statistical significance was observed (p = 0.0629).