Evaluation of laminaritriose hydrolysis.

<p>A. Thin layer chromatographic evaluation of products of laminaritriose hydrolysis at different time points. rOs1BGlu4 (0.125 µg) was incubated with 1 mM laminaritriose in 50 mM sodium phosphate, pH 6.5, at 30 °C from 5 to 30 min (5 m–30 m). Samples were incubated with (+) and without (-) enzyme, then evaluated by silica gel TLC with sulfuric acid staining. The positions of glucose (G); laminaribiose (L2); and laminaritriose (L3) are marked. B: Kinetic data for laminaritriose hydrolysis. The Michaelis–Menten curve and inset Lineweaver-Burk plot are shown, along with the derived kinetic parameters and standard errors.