Blood clearance and biodistribution of annexin-variants in NMRI nude mice.

<p>2-labeled annexin-variant were injected intravenously. At various time-points 25 µl blood samples were collected to measure fluorescence. Blood concentration of annexin-variant was calculated using the constructed reference curves. Panel A shows the blood clearance of anxA5 (grey squares), M1234 (black circles), anxA5-NP (black triangles) and M1234-NP (grey triangles). Half-lives were calculated by mono-exponential fit (B). *p<0.05, **p<0.01, ***p<0.001. 2 nmoles of fluorescently labeled anxA5 (black bars, panel C), M1234 (grey bars, panel C), anxA5-NP (black bars, panel D) and M1234-NP (grey bars, panel D) were injected intravenously into tumor-bearing NMRI nude mice. 1 hour (panel C) and 24 hours (panel D) post-injection organs were collected, weighed and measured for fluorescence. Concentration of annexin-variant were calculated using the constructed reference curves.