PKCδ-dependent S916-phosphorylation of PKD1 precedes PKD1 S744/S748 active loop phosphorylation in N27 dopaminergic cells.

<p>N27 dopaminergic cells were treated with 100 µM 6-OHDA for 1–7 h and monitored for PKD1 S916 phosphorylation (A). N27 cells transiently expressing PKD1^S916A mutant prevent PKD1 activation during oxidative stress, as seen by Western blotting for PKD1 S744/S748 phosphorylation (B).