Modulation of PKD1 offers neuroprotection in N27 dopaminergic neuronal cells.

<p>N27 dopaminergic cells transiently transfected with 5 µg of full-length PKD1 plasmid (PKD1^WT) and 5 µg of vector plasmid were treated with or without 100 µM 6-OHDA and monitored for cytotoxicity at various time points using Sytox green assay. ***, p<0.001 denotes a significant difference between treatment groups from n≥6 (A). N27 dopaminergic cells transiently transfected with 5 µg of constitutively active PKD1^S744E/S748E mutant and 5 µg of vector plasmid were treated with or without 100 µM 6-OHDA and monitored for cytotoxicity at various time points using Sytox green assay. ***, p<0.001 denotes a significant difference between treatment groups from n≥6 (B).