Description of 3D spheroid quantification.

<p>(a–d) Representative pictures of immunolabelled spheroids at t = 0 (a) or after 48 h of culture (c) and their corresponding binarised images (b, d). (e–g) Decomposition of the binarised image into the spheroid core (e) and edging cells (f). (g) Representation of the whole spheroid and its components, the spheroid core (blue) and the edging cells (green). (h) Graph representing the cell density distribution measured from the image. Bars = 500 µm.