Consequences of variants for receptor properties.

<p>Each panel shows a cumulative distribution for the mean parameter values for receptors with different β4 variants. The left graph in each pair shows data for receptors containing the α3 subunit, and the right graph data for receptors containing the α4 subunit. The parameter values have been normalized to the value for wild-type β4 subunits to emphasize the relative functional changes (the actual data are shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0096753#pone-0096753-t002" target="_blank">Tables 2</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0096753#pone-0096753-t003" target="_blank">3</a>). Data for receptors containing wild-type β4 are shown by plus signs, for β4 variants that have PhyloP scores less than 2 (“nonconserved”) by blue filled circles and for those with scores = >2 (“conserved”) by green filled inverted triangles. Hollow symbols show values for variants occurring in only single individuals; these values were not used in the association analyses (see Results). The vertical dashed line shows a relative value of 1 (equal to wild-type). The dashed curves show the cumulative normal distribution predicted by the mean and standard deviation of the data excluding singletons.