Quantitative evaluation of normalized H1R protein expression in the vl VMN and dm VMN.

<p>H1R protein expression levels were quantified for males (n = 5), OVX females (oil injected controls, n = 5), OVX females with EB (5 µg EB, 24 h or 48 h post-injection, n = 5 each), and OVX females with EB+ progesterone (5 µg EB, followed by 500 µg progesterone, n = 5). In the vl VMN, the H1R protein level tended to be increased by EB in OVX females (not statistically significant) and decreased by progesterone. In the dm VMN, hormonal manipulation did not affect H1R protein expression. Data are presented as the mean ±SEM. **, P<0.01. Data are expressed as the ratios of H1R protein to tubulin protein in the vl VMN and dm VMN regions. Since the immunoblotting were obtained independently for each region (from the respective immunoblotting membranes for the vl VMN and dm VMN), data were not to be compared between the two regions.