Activation of AKT–mTOR pathway during mammary tumorigenesis.

<p><b>(a)</b> Western blot analysis of the indicated proteins using whole cell lysates from MCF10A parental (P) or MCF10A NeoT, MCF10AT1, MCF10.DCIS, MCF10 Ca1d, and MCF10 ca1h. Vinculin was used as loading control. Data is representative of three separate experiments. <b>b)</b> Western blot analysis of the indicated proteins using whole cell lysates from MCF10A parental (P) or cyclin E overexpressing cell clone MCF10A-cy E (C3) with and with out IGF1 treatment (25ng/ml for 20minutes). Vinculin was used as loading control. Data is representative of three separate experiments.