SDS-PAGE and Western blot analysis of CHBP in <i>B. pseudomallei</i> K96243 wild-type, <i>chbP</i> mutant and <i>trans</i>-complemented strains.

<p>A) SDS-PAGE. Bacterial lysates and secreted proteins of <i>B. pseudomallei</i> K96243, <i>chbP</i> mutant or <i>chbP</i>/pCHBP strain cultured in LB broth for 6 h were separated by 12% polyacrylamide gel electrophoresis. B) Western blot analysis. The blotted proteins from A) were separately probed with anti-CHBP and anti-BopE antibodies. Molecular mass markers are shown on the left. Lanes 1–3 are bacterial cell lysates and lanes 4–6 are secreted proteins precipitated from culture supernatants.