Rab11 GTPase is not required for MPyV infection.

<p>3T6 cells were transfected with plasmid DNA for transient expression of (A) wild-type EGFP-Rab11 (wt), dominant-negative EGFP-Rab11 (DN) or constitutively active EGFP-Rab11 (CA), or (B) wild-type EGFP-Rab7 (wt), dominant-negative EGFP-Rab7 (DN) or constitutively active EGFP-Rab7 (CA). After 24 h, cells were infected with MPyV, incubated until 24 h p.i., fixed and immunostained for MPyV LT antigen. The efficiency of infection was determined by the levels (%) of LT antigen-positive cells from that expressing EGFP-fused version of the Rab11 or Rab7 GTPase normalized to that obtained in cells expressing its wild-type version. During the experiment, more than 500 cells were counted for each sample or control. Data in the graph represent mean values ± s.d. from three independent experiments.