Nitrate currents in Reissner’s membrane epithelial cells from knockout mice.

<p>A) Whole-cell patch clamp currents from Reissner’s membrane epithelial cells from <i>Slc26a7^Δ/Δ</i> mice were recorded with Cl^–-rich pipette solution and either Cl^–-rich or NO₃^–-rich bath solution. B) The command voltage was held at 0 mV and stepped for 300 ms to +80 mV through −80 mV in 20 mV increments. Representative recordings of step responses before <i>(left)</i> and at the end <i>(right)</i> of the NO₃⁻ bath perfusion are shown. C) Summary of the mean currents at the end of each step is plotted with SEM error bars. Light green panel shows values used for statistical tests of differences in currents and conductances. Green triangles point to the reversal voltages. D) A representative continuous recording during perfusion of Cl^–-rich and NO₃^–-rich bath solutions at the times marked.