TLR2 ligands enhance <i>S. aureus</i>-induced cytokine secretion.

<p><b>A:</b> Adherent human monocytes were stimulated with <i>S. aureus</i> strains: e.g. SA113 and its isogenic mutant Δ<i>lgt</i>, which is deficient in lipoprotein maturation. Secreted IL-6 (left) and TNF (right) concentrations were quantified in the supernatants. The diagrams show the mean values ± SD from cells from six healthy donors. <b>B:</b> HEK293 cells transfected with or without TLR2 plasmid (100 ng/well) were stimulated with <i>S. aureus</i> Lpp SitC (left; 5 ng/ml) or <i>S. aureus</i> strain SA113 (right). (<b>−</b>) refers to unstimulated cells. IL-8 was detected in the supernatants after 24h of stimulation. All diagrams show the mean values ± SD obtained in three experiments.