Reduced plasma IFN-α and increased viral burden in c-FLIP_S female mice infected with CVB3.

<p>(A) IFN-α concentration in plasma from CVB3-infected wild-type and c-FLIP_S female mice. Plasma was obtained from individual mice 7 days after infection and evaluated for IFN-α by ELISA. Results are mean ± SEM. (**significantly different from c-FLIP_S mice at p<0.05). (B) IFN-α production and (C) CVB3 viral titers in T cells <i>in vitro</i>. 10⁴ T lymphocytes from c-FLIP_S and wild-type mice were infected with 10⁵ PFU CVB3 for 30 min and extracellular virus was neutralized using monoclonal anti-CVB3 antibody. The infected cells were cultured for up to 3 days and intracellular viral titers were determined by plaque-forming assay, and IFN-α production determined by ELISA at the times indicated following infection. Results represent mean ± SEM of 4 replicate cultures. (*significantly different from wild type cells at p<0.05).