Salinomycin impairs HCC cell survival.

<p>HepG2 and Huh7 cells were exposed to increasing concentrations of Salinomycin (1, 2, 5 and 10 µM) for 24 hours. (<b>A</b>) Cell viability was assessed by MTS-assay; high concentrations of Salinomycin led to significantly decreased viability of both cell lines (n = 5). (<b>B</b>) HCC cells revealed significantly reduced proliferation after exposure to Salinomycin as demonstrated by decreased ³H-Thymidine uptake. (<b>C</b>) Low concentrations of Salinomycin (left panel, solid line) led to weak increase of apoptotic cells compared to untreated cells (dotted line in overlay). High concentrations markedly induced apoptosis (right panel, solid line). Results are shown as representative scatter-grams of Annexin-V⁺-cells or summarized as mean ± SD of n = 4 independent experiments (<b>D</b>). *p<0.05, **p<0.01.