<i>In vivo</i> validation of the triple reporter components.

<p>Representative live animal images from the coronal plane of six MDA-MB-231 triple reporter tumors and two HT-1080 triple reporter tumors confirm the activity of all three imaging modalities in both cell lines <i>in vivo.</i> The fluorescence signal is shown as the radiant efficiency (p/s/cm²/str)/(mW/cm²). The bioluminescence signal is shown as the radiance photons (p/s/cm²/sr). The PET signal is shown as the% injected dose of ¹⁸F-FHBG per gram. High gut (GI) retention is characteristic of ¹⁸F-FHBG in microPET imaging, which required that tumors be placed away from the abdomen of each mouse.