MiR-21 modulates cell growth via hTERT in HSFBs.

<p>A, the expression of miR-21 was analyzed by qRT-PCR and normalized to U6 in 15 pairs of NSFBs and HSFBs. The 15 paired HSFB/NSFB samples were cultured from 15 individuals. B, HSFBs were transfected with miR-21 mimic, miR-21 inhibitor or a scrambled oligonucleotide. Non-transfected cells (blank) were used as controls. At 24 h post-transfection, miR-21 expression levels in transfected HSFB and NSFB were evaluated by qRT-PCR analysis. C, MTT assays were performed on all four groups at 24 h intervals for 5 days. D, E, The four groups were transfected for 48 h. Apoptosis was measured by flow cytometric analysis of Annexin V and propidium iodide staining. Blank cells were used as control.