Mature human blood cells in the murine liver.

<p>(A) Significantly more light-density liver cells were recovered from 3 mice transplanted with CD34⁺⁺CD38⁻ hFL cells than from untransplanted NSG mice. (B) Myeloid, lymphoid and erythroid engraftment observed 68-166 days after transplantation with hFBM or Lin⁻ LDFL cells. (C) Distribution of T-cell subsets among CD3⁺ T-cells in mice transplanted with hFBM cells. The numbers (n) of animals evaluated are indicate in the 3 box plots. (D) Flow cytometric analysis of light-density liver cells pooled from 3 NSG mice transplanted with CD34⁺⁺CD38⁻ hFL cells were analyzed 144 days after transplantation showing multilineage hematopoietic engraftment. T-cell subsets were evaluated by gating on CD3⁺ cells as indicated. CD56⁺ NK cells were defined by a low side-light scatter gate (not shown) and their lack of CD3 expression. Numbers shown in the graphs represent the percentages of gated events among all CD59⁺ human cells.