Regulation of Lcn2 mRNA expression and secretion by cytokines in adipocytes.

<p>(A) the mRNA expression of Lcn2 in 3T3-L1 adipocytes treated with TNFα, IL-1β, or IL-6 at the concentration of 1 nM for 16 h. The mRNA expression levels in cytokine-treated adipocytes were normalized to the levels in control adipocytes and shown as fold changes. The results are presented as mean ± SE and represent two independent experiments. ** p<0.01, *** p<0.001; * Comparison between control and treated cells. (B) Lcn2 secretion in 3T3-L1 adipocytes treated with TNFα, IL-1β, or IL-6 at the concentration of 1 nM for 24 h. Conditioned media were collected and subjected to immune-blotting with the antibody against Lcn2. The results represent two independent experiments. (C) Western-blotting of Lcn2 in 3T3-L1 adipocytes treated with cytokines under the non-reducing condition.