Analysis of postprandial metabolic and hormonal profile, adipose tissue histology, and mRNA expression in GFP and CPT1AM rats.

<p>Serum and plasma samples from fed GFP and CPT1AM rats were used for all the analyses. (A) Plasma active ghrelin. (B) Serum non-esterified fatty acids (NEFA). (C) Serum leptin. (D) Serum adiponectin. <i>n</i> = 6–10 animals per group. (E) Epididymal WAT histological sections (H & E staining) from representative GFP and CPT1AM rats. (F) White adipocyte diameter scored using ImageJ software and expressed as a fold change respect to GFP animals (5 high power field counted per rat, <i>n</i> = 3 rats per group). (G) mRNA expression of pro-inflammatory markers in WAT from GFP and CPT1AM rats. <i>n</i> = 5–7 animals per group. (H) Interscapular BAT histological sections (H & E staining) from representative GFP and CPT1AM rats. (I) Lipid droplet size scored using ImageJ software and expressed as a fold change respect to GFP animals (4 high power field counted per rat, <i>n</i> = 3 rats per group). (J) mRNA expression of genes associated with lipid metabolism and thermogenesis in BAT from GFP and CPT1AM animals. <i>n</i> = 10 animals per group. Error bars indicate SEM. *<i>p</i><0.05.