Expression of TLR4, TLR6 and TLR13 correlated with the expression of miRNAs that potentially target these transcripts.

<p>Bone marrow cells were cultured as described in Methods and RNA samples were extracted from day 4 to day14 from cells grown in the presence of IL-5. <i>A</i>. Expression levels of TLR4, TLR6 and TLR13 were determined by qPCR. <i>B</i>. Potential miRNAs targeting the 3′-UTR of TLR4, TLR6 and TLR13 were identified by TargetScan, the MiRanda database and IPA ingenuity system. Blue represents decreased expression of miRNAs, whereas yellow is for miRNAs with increased expression. <i>C</i>. The fold changes of potential regulating miRNAs were calculated based on the fluorescence index of each miRNA at different time-points, after normalization to that of the respective miRNAs in the control group (isolated bone marrow cells). Data represent three independent eosinophil cell cultures. Values are presented as mean ±SEM (n = 4∼6), **P<0.001 (vs. d4, d6, d8 or d10).