CD44 independent c-MET activation in HT29 LM3 cells.

<p>(<b>A</b>) Analysis of CD44, p-MET, c-MET, p-AKT and AKT expression in parental HT29 and HT29 LM3 after HGF stimulation (50 ng/mL; 5 min) by Western blot. β-actin was used as a loading control. (<b>B</b>) HT29 LM3 cells were transfected with CD44 siRNA and stimulated with HGF (50 ng/mL; 5 min) 24 h after siRNA transfection. (<b>C</b>) CD44 expression in HT29 LM3 and parental HT29 liver metastasis tissue sections were analyzed by IHC. (<b>D</b>) HT29 LM3 cells were sorted for high and low CD44 expression. Cells were gated and sorted to collect 10% of the cells with high CD44 expression and cells with low CD44 expression. (<b>E</b>) Analysis of CD44, p-MET, and c-MET expression in parental HT29, HT29 LM3 CD44−, and HT29 LM3 CD44+ cells. (<b>F</b>) Analysis of p-MET, c-MET, CD44, p-AKT, and AKT expression in parental HT29, HT29 LM3 CD44−, and HT29 LM3 CD44+ cells after HGF (50 ng/mL; 5 min) stimulation.